汤贤英,孙万邦,罗军敏,宋明英,杜联峰
TANG Xian-ying,SUN Wan-bang,LUO Jun-min,et al(Department of Immunology,Zunyi Medical College

• 1:遵义医学院免疫教研室

摘要(Abstract)：

目的探讨CpG-ODN对伤寒Ty2la疫苗诱导的细胞免疫应答的作用。方法分别用含10、30及50μgCpG-ODN的伤寒Ty21a疫苗免疫小鼠,各剂量组分别于第0、14和28d灌胃免疫3次,末次免疫后1周,采集小鼠脾细胞。流式细胞仪检测脾细胞MHC-Ⅱ类分子和CD40分子的表达;培养脾细胞,用ELISA法检测脾细胞培养上清液中IFN-γ和IL-10的水平。结果不同剂量CpG-ODN的疫苗免疫组脾细胞CD40和MHC-Ⅱ类分子的表达水平均高于阳性对照组,且随着CpG-ODN剂量的增加,表达增强。加入不同剂量CpG-ODN的疫苗免疫组脾细胞培养上清液中IFN-γ和IL-10的水平均升高,且IL-10随着IFN-γ分泌水平的增加而降低。结论CpG-ODN可提高伤寒Ty21a疫苗诱导的Th1型免疫应答水平。
Objective To explore the effect of CpG-ODN on cellular immune response induced by typhoid Ty21a vaccine.Methods NLH mice were immunized with typhoid Ty21a vaccines containing 10,30 and 50 μg of CpG-ODN respectively,by oral route on days 0,14 and 28 d.The splenocytes were collected one week after the third immunization and determined for expressions of MHC-Ⅱ and CD40 molecules by flow cytometry,then cultured and determined for IFN-γ and IL-10 levels in culture supernatant by ELISA.Results The expression levels of MHC-Ⅱ and CD40 molecules in the splenocytes of mice immunized with typhoid Ty21a vaccines containing various concentrations of CpG-ODN were significantly higher than those of positive control,and increased with the increasing CpG-ODN concentration.The IFN-γ and IL-10 levels in culture supernatant of splenocytes of mice immunized with typhoid Ty21a vaccines containing various concentrations of CpG-ODN increased significantly,and the IL-10 level decreased with the increasing IFN-γ level.Conclusion CpG-ODN enhanced the Th1 immune response induced by typhoid Ty21a vaccine.

关键词(KeyWords)： CpG-ODN;佐剂;伤寒Ty21a疫苗;细胞免疫应答
CpG-ODN;Adjuvant;Typhoid Ty21a vaccine;Cellular immune response

Abstract:

Keywords:

基金项目(Foundation): 遵义医学院硕士启动基金(F-139)

作者(Author): 汤贤英,孙万邦,罗军敏,宋明英,杜联峰
TANG Xian-ying,SUN Wan-bang,LUO Jun-min,et al(Department of Immunology,Zunyi Medical College

DOI: 10.13200/j.cjb.2009.02.63.tangxy.018

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