罗红春,张祯祯,张霞,宁波,郭进军,聂娜,刘波,吴小翎
LUO Hong-chun△, ZHANG Zhen-zhen, ZHANG Xia, et al(△Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China)

• 1:重庆医科大学第二附属医院消化内科
• 2:重庆医科大学省部共建感染性疾病分子生物学教育部重点实验室重庆医科大学病毒性肝炎研究所
• 3:重庆医科大学检验实验室

摘要(Abstract)：

目的筛选胃癌中相关miRNAs,并验证其作用靶点。方法应用基因芯片技术检测3份正常胃组织标本、24份胃癌组织标本、胃癌细胞SGC7901和正常胃黏膜细胞GES-1中328个miRNAs的表达情况。采用实时荧光定量PCR对结果进行验证,并用基因克隆和Western blot方法分析miR-9的作用靶点。结果共有26个miRNAs在胃癌标本(包括24份胃癌组织和SGC7901细胞)中异常表达。其中19个下调,7个上调。实时荧光定量PCR检测出miR-9在胃癌标本中的表达水平显著下调,该结果与基因芯片检测结果一致。miR-9与RAS癌基因家族成员RAB34的表达呈负相关。结论已初步筛选了胃癌相关miRNAs。miR-9可能是胃癌中的标记性miRNAs之一,RAB34是其作用靶点。
Objective To screen gastric cancer-associated miRNAs and verify its target. Methods Three normal gastric tis-sue and 24 gastric cancer tissue specimens as well as gastric cancer SGC7901 cells and normal gastric mucosa GES-1 cells were test-ed for expression of 328 miRNAs by microarray technique, and the results were verified by real-time fluorescent quantitative PCR. The target of miR-9 was analyzed by gene cloning and Western blot. Results The expressions of 26 miRNAs were abnormal in gas-tric tissue specimens and SGC7901 cells, of which 19 were down-regulated and 7 were up-regulated. Real-time fluorescent quantita-tive PCR proved that the expression of miR-9 in gastric cancer tissue specimens was down-regulated significantly, which was consistent with the test result by microarray technique. miR-9 was negatively related to the expression of RAB34, a member of RAS gene family. Conclusion Gastric cancer-associated miRNAs were screened primarily, of which miR-9 targeting RAB34 might be one of the marker miRNAs in gastric cancer.

关键词(KeyWords)： 胃癌;微小RNA;miR-9;实时荧光定量PCR;基因芯片
Gastric cancer; miRNAs; miR-9; Real-time fluorescent quantitative PCR; Microarray technique

Abstract:

Keywords:

基金项目(Foundation): 重庆市教委科学技术研究项目(KJ060302)

作者(Author): 罗红春,张祯祯,张霞,宁波,郭进军,聂娜,刘波,吴小翎
LUO Hong-chun△, ZHANG Zhen-zhen, ZHANG Xia, et al(△Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China)

DOI: 10.13200/j.cjb.2009.03.30.luohch.028

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