任萌,张世凯,杨波,王毅,胡征
REN Meng,ZHANG Shikai,YANG Bo,WANG Yi,HU Zheng

• 1:湖北工业大学生物工程与食品学院
• 2:湖北工业大学发酵工程教育部重点实验室

摘要(Abstract)：

目的 制备嗜肺军团菌(Legionella pneumophila,LP)快速检测的胶体金免疫层析试纸，并对其进行性能测试，确保其符合国家临床诊断标准。方法 基于双抗体夹心ELISA法制备LP胶体金免疫层析试纸，并对其交叉反应性、抗干扰性、灵敏度、钩状效应、稳定性等进行测试。结果 LP胶体金免疫层析试纸与呼吸道常见的卡他莫拉菌等22种病原菌均无交叉反应，不受呼吸道内外源干扰物的影响；对LP的最低检测限可达2.00×10~5cfu/m L，具有良好的灵敏度，无钩状效应；在45℃加速老化以及模拟高温运输、冷冻运输条件下，重复性和稳定性均不受影响，且同一批次内、不同批次间试纸稳定性均良好。结论 制备的LP胶体金免疫层析试纸可实现LP的快速检测，操作简便，具有较好的应用前景和推广价值。
ObjectiveTo prepare colloidal gold immunochromatographic test paper for rapid detection of Legionella pneumophila(LP)and test its performance to ensure that it meets the national clinical diagnostic standards.MethodsLP colloidal gold immunochromatographic test paper was prepared based on double antibody sandwich ELISA,and tested for the cross reactivity,anti-interference,sensitivity,hook effect,stability and other aspects.ResultsLP colloidal gold immunochromatography test paper showed no cross reaction with 22 common pathogens in respiratory tract such as Moraxella catarrhalis,and was not affected by internal and external interferences in respiratory tract;The minimum detection limit for LP was 2.00 × 10~5cfu/mL,with good sensitivity and no hook effect;Under the conditions of accelerated aging at 45 ℃,simulated high temperature transportation and frozen transportation,the repeatability and stability of test paper were not affected,and the stability was good in the same batch and between different batches.ConclusionThe prepared LP colloidal gold immunochromatographic test paper realized rapid detection of LP,which was simple to operate and had good application prospect and popularization value.

关键词(KeyWords)： 嗜肺军团菌;胶体金免疫层析试纸;交叉反应性;灵敏度;钩状效应
Legionella pneumophila(LP);Colloidal gold immunochromatographic test paper;Cross reactivity;Sensitivity;Hook effect

Abstract:

Keywords:

基金项目(Foundation): 湖北省科技厅自然科学青年项目(2017CFB226)

作者(Author): 任萌,张世凯,杨波,王毅,胡征
REN Meng,ZHANG Shikai,YANG Bo,WANG Yi,HU Zheng

DOI: 10.13200/j.cnki.cjb.003835

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