于长青,王长远,满永刚,王颖
YU Chang-qing△,WANG Chang-yuan,MAN Yong-gang,WANG Ying(△College of Food Science

• 1:黑龙江八一农垦大学食品学院
• 2:九三集团天津大都科技有限公司

摘要(Abstract)：

目的克隆植物乳杆菌组氨酸脱羧酶(Histidine decarboxylase,HDC)基因,并进行序列分析。方法根据GenBank中登录的乳酸菌HDC基因序列设计引物,提取植物乳杆菌Uvs44基因组DNA进行PCR扩增及克隆,并与其他乳杆菌HDC基因的核苷酸序列进行同源性分析。结果克隆的植物乳杆菌HDC基因大小约为900bp,其核苷酸序列与Lactobacillus sakei同源性最高,为99.9%;与Lactobacillus buchneri的同源性最低,为89.1%。32～489bp为高变区序列,T和C发生的替换较多。结论为产组胺乳酸菌的检测提供了理论依据,也为构建HDC基因缺失工程菌奠定了基础。
Objective To clone Lactobacillus plantrum histidine decarboxylase(HDC)gene and analyze its sequence.Methods Primers were designed according to the lactic acid bacteria HDC gene sequence reported in GenBank,with which the DNA of Uvs44 genome of L.plantrum was extracted,then amplified by PCR and cloned,and analyzed for the homology of nucleotide sequence to that of other lactic acid bacteria.Results The homologies of nucleotide sequence of the cloned HDC gene,at a length of about 900 bp,were 99.9% and 89.1% to those of Lactobacillus sakei and Lactobacillus buchneri respectively.The high variable region of the cloned gene was located at sites 32 ～ 489,in which T and C were often substituted.Conclusion It provided a theoretical basis for the detection of histramine-producing lactic acid bacteria and laid a foundation of construction of HDC gene-deleted recombinant bacterial strain.

关键词(KeyWords)： 植物乳杆菌;组氨酸脱羧酶;克隆,分子;序列分析
Lactobacillus plantrum;Histidine decarboxylase(HDC);Cloning,moloecular;Sequencing

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省教育厅科学技术研究项目(11531258)

作者(Author): 于长青,王长远,满永刚,王颖
YU Chang-qing△,WANG Chang-yuan,MAN Yong-gang,WANG Ying(△College of Food Science

DOI: 10.13200/j.cjb.2010.11.57.yuzhq.008

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