张逸驰,李媛媛
ZHANG Yi-chi,LI Yuan-yuan

• 1:国药中生生物技术研究院有限公司第七研究室

摘要(Abstract)：

杆状病毒是节肢动物特异的DNA病毒,具有双链环状结构,基因排列紧凑,最初因为其高度的特异性,在病虫害防治方面起到了重要作用。1983年人类首次构建杆状病毒载体并成功表达了β-干扰素蛋白,此后,杆状病毒表达载体(baculovirus expression vector,BEV)系统在生产重组病毒、表达重组蛋白、提高重组蛋白稳定性、产量及翻译后修饰等方面不断优化,昆虫细胞-BEV系统成为四大常用表达系统之一。本文主要介绍BEV系统优化最新方法和关键要素。
Baculovirus is an arthropod-specific large circular double-stranded DNA virus,of which the genes are in close array. It plays an important role in pest control because of its high specificity. In 1983,the first recombinant baculovirus vector was constructed,and interferon β protein was successfully expressed. Afterwards,baculovirus expression vector(BEV)system is continuously optimized in the production of recombinant virus as well as expression and improvement of stability,yield and post-translational modification of recombinant protein. Insect cell-baculovirus expression system has become one of the four routine expression systems. This article mainly introduces the novel methods and key elements for optimization of BEV system.

关键词(KeyWords)： 杆状病毒表达系统;蛋白表达;昆虫细胞
Baculovirus expression vector system;Protein expression;Insect cells

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张逸驰,李媛媛
ZHANG Yi-chi,LI Yuan-yuan

DOI: 10.13200/j.cnki.cjb.003232

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