杨春晖,边国慧,阳红,梁启忠,周群刚,李长清
YANG Chun-hui,BIAN Guo-hui,YANG Hong,LIANG Qi-zhong,ZHOU Qun-gang,LI Chang-qing

• 1:中国医学科学院输血研究所
• 2:盐城中心血站
• 3:苏州中心血站

摘要(Abstract)：

目的检测病毒灭活处理血浆中亚甲蓝(methylene blue,MB)残留量。方法将4家采供血机构(A、B、C、D)提供的血浆样品经2个不同型号的一次性病毒灭活装置处理后,采用固相萃取方法分别提取病毒灭活血浆及标准品中MB,利用高效液相色谱法检测MB含量。结果 4家采供血机构病毒灭活血浆MB平均残留量分别为(0.29±0.11)、(0.26±0.09)、(0.09±0.03)和(0.10±0.02)μmol/L。两种不同型号滤器对MB的滤除率分别为91%和73%,差异有统计学意义(P<0.01)。结论目前我国病毒灭活血浆中MB残留量基本符合质量要求,但不同的采供血机构及不同滤器处理的血浆样品间MB残留量仍存在明显差异。
Objective To determine the residual methylene blue(MB)content in plasma after virus inactivation. Methods Plasma samples from four blood centers(A,B,C and D)were treated by disposable virus inactivation apparatuses of two types. The MB in plasma after virus inactivation and standard were extracted by solid phase extraction column,and determined by high performance liquid chromatography(HPLC). Results The residual methylene blue in plasma from four blood centers were(0. 29 ± 0. 11),(0. 26 ± 0. 09),(0. 09 ± 0. 03) and(10 + 0. 02) μmol / L respectively. The removal rates of MB by two types of filters were 91% and 73% respectively,which showed significant difference(P <0. 01). Conclusion The residual MB content in domestic plasma after virus inactivation basically met the requirements for quality control,which showed significant difference in those from various blood centers and those treated by various filters.

关键词(KeyWords)： 血浆;病毒灭活;MB;残留量
Plasma;Virus inactivation;MB;Residual content

Abstract:

Keywords:

基金项目(Foundation): 卫生部行业专项(20092008)

作者(Author): 杨春晖,边国慧,阳红,梁启忠,周群刚,李长清
YANG Chun-hui,BIAN Guo-hui,YANG Hong,LIANG Qi-zhong,ZHOU Qun-gang,LI Chang-qing

DOI: 10.13200/j.cnki.cjb.000863

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