毛颖佳,郑源强,石艳春
MAO Ying-jia,ZHENG Yuan-qiang,SHI Yan-chun(Research Center of Molecular Biology

• 1:内蒙古医学院分子生物学研究中心

摘要(Abstract)：

与其他载体相比,慢病毒载体具有携带基因片段容量大、转染效率高、可感染分裂细胞及非分裂细胞、目的基因可在宿主细胞中长时间稳定表达以及安全性好等诸多优点,现已成为转移目的基因的理想载体。本文主要就慢病毒载体及其在基因治疗、生物医药与科学研究等领域的研究进展作一综述。
Nowadays lentiviral vectors(LVs)is the only desire ideal genetic vector system which affords both efficient and stable gene delivery.In contrast to other vectors,LVs can persist in dividing and non-dividing cells.Their virtues also include large capacity of interested gene fragment and satisfied safety.The development of LVs and their application in gene therapy,biological products and scientific research are reviewed in this paper.

关键词(KeyWords)： 慢病毒载体;基因治疗
Lentiviral vector;Gene therapy

Abstract:

Keywords:

基金项目(Foundation): 教育部“春晖计划”(Z2007-1-01004);; 内蒙古医学院重大项目(NY2006ZD005)

作者(Author): 毛颖佳,郑源强,石艳春
MAO Ying-jia,ZHENG Yuan-qiang,SHI Yan-chun(Research Center of Molecular Biology

DOI: 10.13200/j.cjb.2009.02.97.maoyj.010

参考文献(References)：

• [1]Aris K,Michael T,Oliver G,et al.Discovery and analysis of the first endogenous lentivirus.PNAS,2007,104(15):6261-6265.
• [2]Parolin C,Sodroski J.A defective HIV-1vector for gene transfer to human lymphocytes.J Mol Med,1995,73(6):279-288.
• [3]Curran MA,Kaiser SM,Achacoso PL,et al.Efficient transduction of nondividing cells by optimized feline immunodeficiency virus vectors.Mol Ther,2000,1(1):31-38.
• [4]Mitrophanous K,Yoon S,Rohll J,et al.Stable gene transfer to the nervous system using a non-primate lentiviral vector.Gene Ther,1999,6(11):1808-1818.
• [5]Mselli-Lakhal L,Favier C,Da Silva Teixeira MF,et al.Defective RNA packaging is responsible for low transduction efficiency of CAEV-based vectors.Arch Virol,1998,143(4):681-695.
• [6]Berkowitz R,Ilves H,Lin WY,et al.Construction and molecular analysis of gene transfer systems derived from bovine immunodefi-ciency virus.J Virol,2001,75(7):3371-3382.
• [7]Berkowitz RD,Ilves H,Plavec I,et al.Gene transfer systems de-rived from Visna virus:analysis of virus production and infectivity.Virology,2001,279(1):116-129.
• [8]Poeschla EM.Non-primate lentiviral vectors.Curr Opin Mol Ther,2003,5(5):529-540.
• [9]Cockrell AS,Kafri T.Gene delivery by lentivirus vectors.Mol Biotechnol,2007,36(3):184-204.
• [10]Sumimoto H,Kawakami Y.Lentiviral vector-mediated RNAi and its use for cancer research.Future Oncol,2007,3(6):655-664.
• [11]Liau SS,Ashley SW,Whang EE.Lentivirus-mediated RNA inter-ference of HMGA1promotes chemosensitivity to gemcitabine in pancreatic adenocarcinoma.J Gastrointest Surg,2006,10(9):1254-1263.
• [12]Siming X,Weiyi F,Zhen L.Lentivirus-mediated RNAi silencing targeting ABCC2increasing the sensitivity of a human nasopha-ryngeal carcinoma cell line against cisplatin.J Transl Med,2008,6(1):55-63.
• [13]Bardella C,Dettori D,Olivero M,et al.The therapeutic potential of hepatocyte growth factor to sensitize ovarian cancer cells to cisplatin and paclitaxel in vivo.Clin Cancer Res,2007,13(7):2191-2198.
• [14]Kock N,Kasmieh R,Weissleder R,et al.Tumor therapy mediated by lentiviral expression of shBcl-2and S-TRAIL.Neoplasia,2007,9(5):435-442.
• [15]Bobisse S,Zanovello P,Rosato A.T-cell receptor gene transfer by lentiviral vectors in adoptive cell therapy.Expert Opin Biol Ther,2007,7(6):893-906.
• [16]Yang S,Rosenberg SA,Morgan RA.Clinical-scale lentiviral vec-tor transduction of PBL for TCR gene therapy and potential for expression in less-differentiated cells.Immunother,2008,31(9):830-839.
• [17]Breckpot K,Aerts JL,Thielemans K.Lentiviral vectors for cancer immunotherapy:transforming infectious particles into therapeu-tics.Gene Ther,2007,14(11):847-862.
• [18]Morris KV,Rossi JJ.Anti-HIV-1gene expressing lentiviral vec-tors as an adjunctive therapy for HIV-1infection.Curr HIV Res,2004,2(2):185-191.
• [19]Morris KV,Rossi JJ.Lentiviral-mediated delivery of siRNAs for antiviral therapy.Gene Ther,2006,13(6):553-558.
• [20]Joseph A,Zheng JH,Follenzi A,et al.Lentiviral vectors encoding human immunodeficiency virus type1(HIV-1)-specific T-cell re-ceptor genes efficiently convert peripheral blood CD8T lympho-cytes into cytotoxic T lymphocytes with potent in vitro and in vivo HIV-1-specific inhibitory activity.J Virol,2008,82(6):3078-3089.
• [21]Salmon P,Trono D.Production and titration of lentiviral vectors.Curr Protoc Neurosci,2006,Chapter4:Unit4.21.
• [22]Wong LF,Goodhead L,Prat C,et al.Lentivirus-mediated genetransfer to the central nervous system:therapeutic and research applications.Hum Gene Ther,2006,17(1):1-9.
• [23]Sinn PL,Burnight ER,Hickey MA,et al.Persistent gene expres-sion in mouse nasal epithelia following feline immunodeficiency virus-based vector gene transfer.J Virol,2005,79(20):12818-12827.
• [24]McKay T,Patel M,Pickles RJ,et al.Influenza M2envelope pro-tein augments avian influenza hemagglutinin pseudotyping of lentiviral vectors.Gene Ther,2006,13(8):715-724.
• [25]Haynes JR,Dokken L,Wiley JA,et al.Influenza-pseudotyped Gag virus-like particle vaccines provide broad protection against highly pathogenic avian influenza challenge.Vaccine,2009,27(4):530-541.
• [26]石艳春,梁浩,旭日干.融合表达IL-2和EGFP逆转录病毒载体的构建.中国生物制品学杂志,2006,19(1):1-4.
• [27]Fu Y,Luo L,Luo N,et al.Proinflammatory cytokine production and insulin sensitivity regulated by overexpression of resistin in3T3-L1adipocytes.Nutr Metab(Lond),2006,3(1):28-37.
• [28]Virk MS,Conduah A,Park SH,et al.Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2expression on the quality of bone repair in a rat femoral defect model.Bone,2008,42(5):921-931.
• [29]Lillico SG,Sherman A,McGrew MJ,et al.Oviduct-specific ex-pression of two therapeutic proteins in transgenic hens.Proc Natl Acad Sci USA.2007,104(6):1771-1776.
• [30]Pestina TI,Hargrove PW,Jay D,et al.Correction of murine sick-le cell disease using gamma-globin lentiviral vectors to mediate high-level expression of fetal hemoglobin.Mol Ther,2008,2.
• [31]Niemann H,Kues WA.Transgenic farm animals:an update.Re-prod Fertil Dev,2007,19(6):762-770.
• [32]Park F.Lentiviral vectors:are they the future of animal transgen-esis.Physiol Genomics,2007,31(2):159-173.
• [33]Baba K,Goto-Koshino Y,Mizukoshi F,et al.Inhibition of the replication of feline immunodeficiency virus by lentiviral vector-mediated RNA interference in feline cell lines.J Vet Med Sci,2008,70(8):777-783.
• [34]Joo KI,Wang P.Visualization of targeted transduction by engi-neered lentiviral vectors.Gene Ther,2008,15(20):1384-1396.
• [35]Semple-Rowland SL,Eccles KS,Humberstone EJ.Targeted ex-pression of two proteins in neural retina using self-inactivating,insulated lentiviral vectors carrying two internal independent promoters.Mol Vis,2007,13:2001-2011.
• [36]Hargrove PW,Kepes S,Hanawa H,et al.Globin lentiviral vector insertions can perturb the expression of endogenous genes in be-ta-thalassemic hematopoietic cells.Mol Ther,2008,16(3):525-533.
• [37]Bauer G,Dao MA,Case SS,et al.In vivo biosafety model to as-sess the risk of adverse events from retroviral and lentiviral vec-tors.Mol Ther,2008,16(7):1308-1315.