王丽梅,王鑫,胡伟,詹建萍,吕淑敏,张永明
WANG Limei,WANG Xin,HU Wei,ZHAN Jianping,LV Shumin,ZHANG Yongming

• 1:内蒙古师范大学生命科学与技术学院
• 2:内蒙古大溪生物科技有限公司

摘要(Abstract)：

目的 比较含血小板裂解液（platelet lysate,PL）的新生牛血清（newborn calf serum,NBCS）与胎牛血清（fetal bovine serum,FBS）培养BHK-21、Vero、HEK293T细胞的促生长效果，为探究PL-NBCS替代FBS培养细胞的可行性提供实验依据。方法 分别用PL-NBCS和FBS培养BHK-21、Vero和HEK293T细胞，通过对连续传代培养细胞的形态观察、细胞平均增殖密度、活率、倍增时间等动力学分析检测PL-NBCS对细胞的促生长作用。结果 PL-NBCS培养的3种细胞形态特征与FBS培养的细胞无显著差异。在两种培养条件下，细胞生长均呈“S”型。PL-NBCS与FBS培养的BHK-21细胞平均增殖密度、活率、倍增时间差异均无统计学意义（t分别为0.734、0.432和0.029,P分别为0.472、0.671和0.977）;PL-NBCS培养的Vero细胞平均增殖密度和活率均高于FBS组，且平均倍增时间显著高于FBS组（t=2.228,P=0.042）;PL-NBCS培养的HEK293T细胞平均增殖密度、活率及倍增时间均显著高于FBS组（t分别为2.309、2.848和2.682,P分别为0.033、0.011和0.015）。结论 PL-NBCS培养细胞促生长效果较好，可替代FBS传代培养细胞。
Objective To compare the growth-promoting effects of platelet lysate-containing newborn calf serum(PL-NBCS) and fetal bovine serum(FBS) on cultured BHK-21,Vero,HEK293T cells,and to provide experimental basis for exploring the feasibility of culturing cells with PL-NBCS instead of FBS.Methods BHK21 cells,Vero cells and HEK293T cells were cultured with PL-NBCS and FBS,respectively.The growth-promoting effect of PL-NBCS on cells was detected by kinetic statistical analysis of morphological observation,average cell proliferation density,viability and doubling time of continuously subcultured cells.Results There was no significant difference in morphological characteristics between three kinds of cells cultured in PL-NBCS and those cultured in FBS.Under the two culture conditions,the cells grew in an "S" shape.The average proliferation density,viability and doubling time of BHK-21 cells cultured in PL-NBCS showed no significant difference with the BHK-21 cells cultured in FBS(t=0.734,0.432 and 0.029,P=0.472,0.671 and 0.977,respectively).The average proliferation density and viability of Vero cells cultured in PL-NBCS were higher than those in FBS group,and the average doubling time was significantly higher than that in FBS group(t=2.228,P=0.042).The average proliferation density,viability and doubling time of HEK293T cells cultured in PL-NBCS were significantly higher than those in FBS group(t=2.309,2.848 and 2.682,P=0.033,0.011 and 0.015,respectively).Conclusion PL-NBCS has better growth-promoting effect on cells,and can replace FBS to subculture cells.

关键词(KeyWords)： 血小板裂解液;新生牛血清;胎牛血清;悬浮细胞培养;贴壁细胞培养
Platelet lysate(PL);Newborn calf serum(NBCS);Fetal bovine serum(FBS);Suspension cell culture;Adherent cell culture

Abstract:

Keywords:

基金项目(Foundation): 内蒙古自治区科技创新引导项目(kcjl-202218);; 内蒙古自治区科技计划项目(2023YFHH0066);; 呼和浩特市科技创新领域人才项目(2023RC-联合体-4)

作者(Author): 王丽梅,王鑫,胡伟,詹建萍,吕淑敏,张永明
WANG Limei,WANG Xin,HU Wei,ZHAN Jianping,LV Shumin,ZHANG Yongming

DOI: 10.13200/j.cnki.cjb.004499

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