方立超,魏泓

• 1:第三军医大学医学检验系临床检验学教研室
• 2:第三军医大学基础部实验动物学教研室 (重庆400038)
• 3:(重庆400038)

摘要(Abstract)：

益生菌对人体有多种保健作用,本文就益生菌定义演变、菌株选择标准、研究方法、常用菌株、存在问题及前景进行了综述。

关键词(KeyWords)： 益生菌;保健

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 方立超,魏泓

DOI: 10.13200/j.cjb.2007.06.76.fanglch.022

参考文献(References)：

• [1]Working group report.Guidelines for the Evaluation of Probiotics in Food,Joint Food and Agriculture Organisation of the United Nations and The World Health Organisation.2007.
• [2]Marteau P,Shanahan F.Basic aspects and pharmacology of probiot-ics:an overiewof pharmacolinetics,mechanisms of action and side-effects.Best Pract Res Clin Gastroenterol,2003,17(5):725-740.
• [3]Holzapfel WH,Haberer P,Snel J,et al.Overview of gut flora and probiotics.Int J Food Microbiol,1998,41(2):85-101.
• [4]Salmine S,Isolauri E.Clinical applications of probiotic bacteria.Int Dairy J,1998,8(5-6):563-572.
• [5]Alander M,Korpela R,Saxelin M,et al.Recovery of Lactobacillus rhamnosus GG from human colonic biopsies.Lett Appl Microbiol,1997,24(5):361-364.
• [6]Elliott SN,Buret A,McKnight W,et al.Bacteria rapidly colonize and modulate healing of gastric ulcers in rats.Am J Physiol,1998,275(3):425-432.
• [7]Reid G,Burton J.Use of Lactobacillus to prevent infection by patho-genic bacteria.Microbes Infect,2002,4(3):319-324.
• [8]Blum S,Haller D,Pfeifer A,et al.Probiobics and immune response.Clin Rev Allergy Immunol,2002,22(3):287-309.
• [9]Lesuffleur T,Barbat A,Dussaulx E,et al.Growth adaptation to methotrexate of HT-29human colon carcinoma cells is associated with their ability of differentiate into columnar absorptive and mucus-secreting cells.Cancer Res,1990,50(19):6334-6343.
• [10]Lesuffleur T,Porchet N,Aubert JP,et al.Differential expression of the human mucin genes MUC1to MUC5in relation to growth and differentiation of different mucus-secreting HT-29cell subpopula-tions.J Cell Sci,1993,106(Pt3):771-783.
• [11]Kiely LJ,Olson NF.The physicochemical surface characteristics of Lactobacillus casei.Food Microbiol,2000,17(3):277-291.
• [12]Athamna A,Ofek I.Enzyme-linked immunosorbant assay for quan-titation of attachment and ingestion stages of bacterial phagocytosis.J Clin Microbiol,1988,26(1):62-66.
• [13]Ofek I,Courtney HS,Schifferli DM,et al.Enzyme-linked immu-nosorbant assay for adherence of bacteria to animal cells.J Clin Mi-crobiol,1986,24(4):512-516.
• [14]Gwena lle LB,Isma l F,Christophe L.Comparative detection of bacterial adhesion to Caco-2cells with ELISA,radioactivity and plate count methods.J Microbiol Methods,2004,59(2):211-221.
• [15]Ahrne S,Nobaek S,Jeppsson B,et al.The normal Lactobacillus flora of healthy human rectal and oral mucosa.J Appl Microbiol,1998,85(1):88-94.
• [16]De Keersmaecker SCJ,Braeken K,Verhoeven TLA,et al.Flow cytometric testing of green fluorescent protein-tagged Lactobacillus rhamnosus GG for response to defensins.Appl Environ Microbiol,2006,72(7):4923-4930.
• [17]Geoffroy MC,Gurard C,Quatannens B,et al.Use of green fluo-rescent protein to tag lactic acid bacteriumstrains under development as live vaccine vectors.Appl Environ Microbiol,2000,66(1):383-391.
• [18]Klijn N,Weerkamp AH,De Vos WM.Genetic marking of lacto-coccus lactis shows its survival in the human gastrointestinal tract.Appl Environ Microbiol,1995,61(7):2771-2774.
• [19]Fernandez L,Marin ML,Langa S,et al.A novel genetic label for detection of specific probiotic lactic acid bacteria.Food Sci Technol Int,2004,10(2):101-108.
• [20]Vaughan EE,Schut F,Heiling HGHJ,et al.A molecular view of the intestinal ecosystem.Curr Issues Intest Microbiol,2000,1(1):1-12.
• [21]Sheridan CEC,Masters CI,Shallcross JA,et al.Detection of mRNA by reverse transcription-PCR as an indicator of viability inEscherich-ia colicells.Appl Environ Microbiol,1998,64(4):1313-1318.
• [22]Yasuo S,Mitsuo S,Satoru T,et al.Monitoring the cell number and viability of Lactobacillus helveticus GCL1001in human feces by PCR methods.FEMS Microbiol Lett,2004,231(1):125-130.
• [23]Marteau P,Vesa T.Pharmacokinetics of probiotics and biothera-peutic agents in humans.Biosci Microflora,1998,17(1):1-6.
• [24]Shah NP.Probiotic bacteria:selection enumeration and survival in dairy foods.J Dairy Sci,2000,83(4):894-907.
• [25]Bruno-Bárcena JM,Andrus JM,Libby SL,et al.Expression of a heterologous manganese superoxide dismutase gene in intestinal lac-tobacilli provides protection against hydrogen peroxide toxicity.Appl Environ Microbiol,2004,70(8):4702-4710.
• [26]Liu JR,Yu B,Liu FH,et al.Expression of rumen microbial fibro-lytic enzyme genes in probioticLactobacillus reuteri.Appl Environ Microbiol,2005,71(11):6769-6775.