流行性乙型脑炎病毒E蛋白上与病原性相关的氨基酸Pathogenicity-related Amino Acid on Protein E Of Japanese Encephalitis Virus
李玉华,胁田隆,保井孝太郎
LI Yuhua;Wakita Zisi;Yasui Kotano et al ( Chengdu;
摘要(Abstract)：
目的 分析比较乙脑病毒弱毒株at222与其强毒株AT31全基因核苷酸序列，探索与乙脑病毒病原性相关的氨基酸。方法 由日本基因公司合成多核苷引物，以RT-PCR合成cDNA，筛选出阳性克隆。用Sanger法测定at222与AT31株的全基因序列。结果 全基因序列分析比较显示，非编码区3个不同核苷苷酸均出现在3’－端。编码蛋白氨基酸变化分别是E蛋白上3个，NSI蛋白上3个，NS蛋白上2个，NS4a蛋白上1个，NS4b蛋白上1个，NS5蛋白上2个。结论 强弱毒株间2个变异部位与以前报道一致，且其中AT31的E138位点上E氨基酸为强毒株JaGAr01、JaOArS982、Beijing－l和SA14所共有，而at222E138位点上K氨基酸为弱毒株SA14－14－2所共有。E蛋白上E138、E176位点上氨基酸决定了乙脑病毒病原性。
Abethect: Objective To analyze and compare the nucleotide sequences of whole gene of at222(weak) and AT31(strong) strains of Japanese encephalitis (JE) virus and explore the amino acids reIated tothe pathogenicity of JE virus. Methods The cDNAs of at222 and AT31 strains were synthesized by RT -PCR using designed primer and tranaformed to E. coli, and the pesitive colonies were selected out and se-quenced by Sanger Method. Results Both strains AT31 and at222 showed 3 nucleotide variants in non -coding regions at the 3' - terminals. Amino acid variants were observed in the protein E (3), NSl (3), NS3(2), NS4a (1), Nsab (1) and NS5 (2) of the 2 strains separately. Conclusion The 2 variant sites be-tween strains at222 and AT31 were consistent with those reported previously. The amoino acid E at the siteE138 of AT31 was common to strong strains TaCAr01, JaOArS982, Beijing - 1 and SA14. However, the ami-no acid K at the site E138 of at222 was also common to weak stain SA14 - 14 - 2. So the pathogenicity of JEvirus depend upon the drino acids at the sites E138 and E176 at protein E.
关键词(KeyWords)：
流行性乙型脑炎;病原性;氨基酸
Janpanese encephalitis Pathogenicity Amino acid
基金项目(Foundation):
作者(Authors):
李玉华,胁田隆,保井孝太郎
LI Yuhua;Wakita Zisi;Yasui Kotano et al ( Chengdu;
DOI: 10.13200/j.cjb.2002.01.7.liyh.002
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