柴政斌,张更林,韩金祥
CHAI Zheng-bin,ZHANG Geng-lin,HAN Jin-xiang

• 1:卫生部生物技术药物重点实验室山东省罕少见病重点实验室山东省医药生物技术研究中心山东省医学科学院
• 2:济南大学山东省医学科学院医学与生命科学学院

摘要(Abstract)：

蛋白间相互作用在细胞的生命活动中起着关键作用,GST-pull down技术被广泛用于体外验证蛋白间的直接相互作用。GST-pull down技术既可用来验证已知蛋白间的相互作用,也可用来寻找与已知蛋白相互作用的未知蛋白。虽然该技术在体外验证蛋白间的相互作用上有着较强的特异性,但下结论时仍需慎重。本文就GST-pull down技术的原理及其应用及该技术在应用中需要注意的部分问题进行综述。
Protein-protein interaction play an pivotal role in the vital movement of cells. Glutathione-S-transferase(GST)-pull down assay is widely used for the verification of direct protein-protein interaction in vitro,including those between the known proteins and those between known and unknown proteins. Although GST-pull down assay has a strong specificity in verification of direct protein-protein interaction in vitro,the conclusion should be given with caution. This paper reviews the principle and application as well as some problems during application of GST-pull down assay.

关键词(KeyWords)： GST-pull down;蛋白质相互作用;融合蛋白
GST-pull down;Protein-protein interaction;Fusion protein

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 柴政斌,张更林,韩金祥
CHAI Zheng-bin,ZHANG Geng-lin,HAN Jin-xiang

DOI: 10.13200/j.cnki.cjb.000628

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